{"id":4960,"date":"2019-04-20T08:36:39","date_gmt":"2019-04-20T08:36:39","guid":{"rendered":"https:\/\/www.elch.chem.msu.ru\/wp3\/?p=4960"},"modified":"2019-04-20T21:36:41","modified_gmt":"2019-04-20T18:36:41","slug":"200419","status":"publish","type":"post","link":"https:\/\/www.elch.chem.msu.ru\/wp3\/index.php\/ru\/2019\/04\/20\/200419\/","title":{"rendered":"\u0441\u0435\u043c\u0438\u043d\u0430\u0440 \u0432 \u0438\u0444\u0445\u044d, 23 \u0430\u043f\u0440\u0435\u043b\u044f"},"content":{"rendered":"
\u0414\u043e\u0440\u043e\u0433\u0438\u0435 \u043a\u043e\u043b\u043b\u0435\u0433\u0438,<\/span><\/div>\n
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\u0412\u043e \u0432\u0442\u043e\u0440\u043d\u0438\u043a, 23 \u0430\u043f\u0440\u0435\u043b\u044f, \u0441\u043e\u0441\u0442\u043e\u0438\u0442\u0441\u044f \u043e\u0447\u0435\u0440\u0435\u0434\u043d\u043e\u0439 \u0441\u0435\u043c\u0438\u043d\u0430\u0440 \u043b\u0430\u0431\u043e\u0440\u0430\u0442\u043e\u0440\u0438\u0438. \u0421 \u0434\u043e\u043a\u043b\u0430\u0434\u043e\u043c \u00abElectrochemiluminescence microscopy confined in nanofluidic devices and at the electrode surface for cell imaging\u00bb \u0432\u044b\u0441\u0442\u0443\u043f\u0438\u0442 Neso Sojic (University of Bordeaux). \u041d\u0430\u0447\u0430\u043b\u043e \u0441\u0435\u043c\u0438\u043d\u0430\u0440\u0430 \u0432 15:00 \u0432 \u043a.253 \u0418\u0424\u0425\u042d \u0420\u0410\u041d.<\/span><\/p>\n

\u0415\u0441\u043b\u0438 \u0432\u0430\u043c \u043d\u0443\u0436\u0435\u043d \u043f\u0440\u043e\u043f\u0443\u0441\u043a, \u043f\u043e\u0436\u0430\u043b\u0443\u0439\u0441\u0442\u0430, \u043d\u0430\u043f\u0438\u0448\u0438\u0442\u0435 \u0437\u0430\u0440\u0430\u043d\u0435\u0435 (\u0434\u043e 23 \u0430\u043f\u0440\u0435\u043b\u044f) \u0422\u0430\u0440\u0430\u0441\u0443 \u041c\u043e\u043b\u043e\u0442\u0438\u043b\u0438\u043d\u0443<\/a> <\/span><\/p>\n

ABSTRACT:<\/span><\/p>\n

Microfabricated nanofluidic electrochemical devices offer a highly controlled nanochannel geometry; they confine the volume of chemical reactions to the nanoscale and enable greatly amplified electrochemical detection by redox cycling. <\/span>In a first part, the generation of stable light emission by ECL in transparent nanofluidic devices (see fig. below) will be presented by exploiting nanogap amplification. Through continuous oxidation and reduction of [Ru(bpy)3]2+ luminophores at electrodes positioned at opposite walls of a 100-nm nanochannel, we compared classic redox cycling and ECL annihilation.[1] Enhanced ECL light emission of attomole luminophore quantities was evidenced under ambient conditions due to the spatial confinement in a 10-femtoliter volume, resulting in a short diffusion timescale and highly efficient ECL reaction pathways at the nanoscale.<\/span><\/p>\n

In a second part, a surface-confined microscopy based on ECL which allows to image the plasma membrane of single cells at the interface with an electrode. By analyzing photoluminescence (PL), ECL and AFM images of mammalian CHO cells, we demonstrated that, in contrast to the wide-field fluorescence, ECL emission is confined to the immediate vicinity of the electrode surface and only the basal membrane of the cell becomes luminescent.[2-3] The resulting ECL microscopy reveals details, which are not resolved by classic fluorescence microscopy, without any light irradiation and specific setup. Our approach opens new avenues for ECL as a surface-confined microscopy to image biological entities in cells and to develop circularly polarized\u2010ECL methods.[4]<\/span><\/p>\n

[1] H. Al-Kutubi, S. Voci, L .Rassaei, N. Sojic, K. Mathwig. Chem .Sci., 9, 2018, 8946-8950<\/span><\/p>\n

[2] G. Valenti, S. Scarabino, B. Goudeau, A. Lesch, M. Jovic, E. Villani, M. Sentic, S. Rapino, S. Arbault, F. Paolucci, N. Sojic. J. Am. Chem. Soc., 139, 2017, 16830\u201316837<\/span><\/p>\n

[3] S. Voci, B. Goudeau, G. Valenti, A. Lesch, M. Jovi\u0107, S. Rapino, F. Paolucci, S. Arbault, N. Sojic. <\/span>J. Am. Chem. Soc., 140, 2018, 14753-14760<\/span><\/p>\n

[4] F. Zinna, S. Voci, L. Arrico, E. Brun, A. Homberg, L. Bouffier, T. Funaioli, J. Lacour, N. Sojic, L.Di Bari. <\/span>Angew. Chem. Int. Ed., 2019, DOI : 10.1002\/anie.201901303<\/span><\/p>\n

\u00a0<\/span><\/p>\n

\u00a0<\/span><\/p>\n

\u041e.\u0418.\u0412\u0438\u043d\u043e\u0433\u0440\u0430\u0434\u043e\u0432\u0430<\/span><\/p>\n

\u00a0<\/span><\/p>\n

\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

\u0414\u043e\u0440\u043e\u0433\u0438\u0435 \u043a\u043e\u043b\u043b\u0435\u0433\u0438, \u0412\u043e \u0432\u0442\u043e\u0440\u043d\u0438\u043a, 23 \u0430\u043f\u0440\u0435\u043b\u044f, \u0441\u043e\u0441\u0442\u043e\u0438\u0442\u0441\u044f \u043e\u0447\u0435\u0440\u0435\u0434\u043d\u043e\u0439 \u0441\u0435\u043c\u0438\u043d\u0430\u0440 \u043b\u0430\u0431\u043e\u0440\u0430\u0442\u043e\u0440\u0438\u0438. \u0421 \u0434\u043e\u043a\u043b\u0430\u0434\u043e\u043c \u00abElectrochemiluminescence microscopy confined in nanofluidic devices and at the electrode surface for cell imaging\u00bb \u0432\u044b\u0441\u0442\u0443\u043f\u0438\u0442 Neso Sojic (University of Bordeaux). \u041d\u0430\u0447\u0430\u043b\u043e \u0441\u0435\u043c\u0438\u043d\u0430\u0440\u0430 \u0432 15:00 \u0432 \u043a.253 \u0418\u0424\u0425\u042d \u0420\u0410\u041d. \u0447\u0438\u0442\u0430\u0442\u044c \u0434\u0430\u043b\u044c\u0448\u0435 …<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[30],"tags":[],"class_list":["post-4960","post","type-post","status-publish","format-standard","hentry","category-news"],"_links":{"self":[{"href":"https:\/\/www.elch.chem.msu.ru\/wp3\/index.php\/wp-json\/wp\/v2\/posts\/4960","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.elch.chem.msu.ru\/wp3\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.elch.chem.msu.ru\/wp3\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.elch.chem.msu.ru\/wp3\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.elch.chem.msu.ru\/wp3\/index.php\/wp-json\/wp\/v2\/comments?post=4960"}],"version-history":[{"count":3,"href":"https:\/\/www.elch.chem.msu.ru\/wp3\/index.php\/wp-json\/wp\/v2\/posts\/4960\/revisions"}],"predecessor-version":[{"id":4965,"href":"https:\/\/www.elch.chem.msu.ru\/wp3\/index.php\/wp-json\/wp\/v2\/posts\/4960\/revisions\/4965"}],"wp:attachment":[{"href":"https:\/\/www.elch.chem.msu.ru\/wp3\/index.php\/wp-json\/wp\/v2\/media?parent=4960"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.elch.chem.msu.ru\/wp3\/index.php\/wp-json\/wp\/v2\/categories?post=4960"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.elch.chem.msu.ru\/wp3\/index.php\/wp-json\/wp\/v2\/tags?post=4960"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}